ABSTRAK
Getah Jarak tintir (Jatropha multifida Linn.) memiliki aktivitas antioksidan yang
berpotensi sebagai antihiperpigmentasi dalam bentuk sediaan topikal. Kulit bersifat
impermeable sehingga sulit ditembus karena lapisan stratum corneum dikelilingi
keratin dan lapisan lipid interseluler sehingga digunakan sistem penghantaran obat
dalam bentuk Nanostructured Lipid Carriers (NLC). Penelitian bertujuan untuk
menentukan serbuk getah Jarak tintir dapat dibuat menjadi sistem NLC dan
kemampuan penetrasi in vitro kedalam kulit. Sistem NLC dibentuk menggunakan
metode High Shear Homogenization (HSH) dengan konsentrasi lipid 8% dan
surfaktan 20% kemudian dilakukan karakterisasi NLC dan penentuan kadar
terpenetrasi menggunakan sel difusi Franz selama 8 jam. Hasil penelitian
menunjukkan serbuk getah Jarak tintir memiliki nilai IC
50
sebesar 4,93±0,18 µg/mL
kategori antioksidan sangat kuat, nilai kadar flavonoid total sebesar 4,5573±0,02
mgQE/g serta dapat dibentuk menjadi sistem NLC. Karakterisasi sistem NLC
menghasilkan ukuran partikel 94,2±3,60 nm, indeks polidispersi 0,386±0,06,
potensial zeta 0,7±0,09 mV, efisiensi penjerapan 71,03±0,66% dan pengamatan
morfologi menggunakan SEM dengan perbesaran 30x menunjukkan permukaan
yang tidak merata serta berongga. Sistem NLC memiliki stabilitas kurang baik
selama penyimpanan dibandingkan sediaan krim. Kadar flavonoid total yang
terpenetrasi pada F1 sebesar 68,297±0,26 µg/cm
2
dan F2 sebesar 42,332±0,44
µg/cm
2
sedangkan persen terpenetrasi F1 paling besar yaitu 13,235±0,05 % dan F2
yaitu 5,831±0,06 % pada menit ke-480. Hasil menunjukkan bahwa sistem NLC
memiliki kemampuan penetrasi flavonoid total dua kali lipat lebih baik
dibandingkan sediaan krim.

Kata kunci : getah jarak tintir, antioksidan, Nanostructured Lipid Carrier, ukuran
partikel, sel difusi Franz

ABSTRACT
The latex of Jatropha Tintir (Jatropha multifida Linn.) has antioxidant activity that
has potential as an antihyperpigmentation in topical dosage form. The skin is
impermeable so it is difficult to penetrate because the stratum corneum layer is
surrounded by keratin and intercellular lipid layers so that drug delivery systems
are used in the form of Nanostructured Lipid Carriers (NLC). The study aimed to
determine the Colorir castor sap powder can be made into NLC systems and the
ability to penetrate in vitro into the skin. The NLC system was formed using the
High Shear Homogenization (HSH) method with a lipid concentration of 8% and
surfactant of 20%, then NLC characterization and determination of penetrated
levels using Franz diffusion cells for 8 hours. The results showed that Jatropha
Tintir sap powder has an IC50 value of 4.93±0.18 μg/mL in the very strong
antioxidant category, a total flavonoid content value of 4.5573±0.02 mgQE/g and
can be formed into an NLC system. NLC system characterization resulted in a
particle size of 94.2±3.60 nm, polydispersion index 0.386±0.06, zeta potential
0.7±0.09 mV, absorption efficiency 71.03±0.66% and morphological observations
using SEM with 30x magnification showing an uneven and hollow surface. NLC
systems have poor stability during storage than cream preparations. Total flavonoid
levels penetrated in F1 amounted to 68.297±0.26 μg/cm
2
and F2 amounted to
42.332±0.44 μg/cm
2
while the percent penetrated by F1 was greatest at
13.235±0.05% and F2 was 5.831±0.06% in the 480th minute. The results showed
that the NLC system had twice the total flavonoid penetration ability compared to
cream preparations.

Keywords : the latex of jatropha tintir, antioxidant, Nanostructured Lipid Carrier,
particle size, Franz diffusion cell